55 urn:lsid:arphahub.com:pub:8FE60005-0CE8-5F90-AF8B-9EF8C18A0BCA BioDiscovery BD 2050-2966 Pensoft Publishers 10.3897/biodiscovery.20.e19830 19830 7885 Conference Abstract World BioDiscovery Congress 2017 Proceedings Ultrananocrystalline diamond coated implants for enhanced osseointegration Stateva Silviya R 1 Merker Daniel 2 Popov Cyril 2 Apostolova Margarita margo@obzor.bio21.bas.bg https://orcid.org/0000-0001-7188-506X 1 Roumen Tsanev Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria Roumen Tsanev Institute of Molecular Biology, Bulgarian Academy of Sciences Sofia Bulgaria Institute of Nanostructure Technologies and Analytics, Center for Interdisciplinary Nanostructure Science and Technology, University of Kassel, Kassel, Germany Institute of Nanostructure Technologies and Analytics, Center for Interdisciplinary Nanostructure Science and Technology, University of Kassel Kassel Germany

Corresponding author: Margarita Apostolova (margo@obzor.bio21.bas.bg).

Academic editor:

2017 01 08 2017 20 e19830 FFC6FFB9-FFB5-A94F-4C63-0675B111FFC4 1150196 26 07 2017 Silviya R Stateva, Daniel Merker, Cyril Popov, Margarita Apostolova This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Osteoporosis causes bones to become weak and brittle. It is known that the alterations in bone metabolism associated to osteoporosis can impair bone healing around implants and affect their osseointegration. The main objective of this study was the development of new nanostructured implant materials based on ultrananocrystalline diamond (UNCD) coatings for enhancing osseointegration. The films were deposited on Ti substrates by microwave plasma CVD from 17% CH4/N2 gas mixtures and modified by O2 or NH3/N2 plasmas. The modifications rendered the H-terminated hydrophobic as-grown films hydrophilic. The interaction of endothelial (EA.hy926) and osteosarcoma (MG63) cells with differently modified UNCD surfaces was investigated by proteome analyses. It revealed the identification of over 19 000 proteins (extracellular and cytosolic). They correspond to 508 (Ti), 634 (UNCD), 651 (O-UNCD), and 668 (NH2-UNCD) protein groups. The interaction network analysis showed differences in the connectivity of inferred protein networks between the ECM niches, which suggests the presence of specific cell microenvironments on O- and NH2-terminated UNCD surfaces. Our results show that due to a favorable combination of surface chemical and topological properties the UNCD films, as-grown and especially after their plasma modifications, may serve as superior scaffolding for promoting the cell attachment and growth during osseointegration.

 Ultrananocrystalline diamond films Surface modification Titanium Implant materials Osseointegration Proteomics Presenting author

Silviya R Stateva

 Presented at

World BioDiscovery Congress 2017

 Conflicts of interest

The authors declare that there is no conflict of interests.

 Acknowledgements

The authors are grateful to the Deutsche Forschungsgemeinschaft (DFG) for the financial support under the Project PO 789/3-1 and DAAD / Bulgarian NSF for the support of the bilateral cooperation (DNTS - Germany 01/7); Project BG161PO003-1.2.04-0013-C0001 co-financed by the EU through the European Regional Development Fund and the State Budget of the Republic of Bulgaria.