Endogenous RAGE mRNA and protein expression in breast cancer cell lines. Panel A The expression of RAGE was examined at mRNA level by quantitative RT-PCR (gray) and at protein level by western blot analysis (white). AU stands for arbitrary units. The data are normalized to beta actin. The value of 1 corresponds to the respective levels of RAGE mRNA and protein in MCF-10A. The data represent the mean SD (n = 3). Panel B A representative western blot. Panel C Immunolocalization using an anti-RAGE antibody (1:500 dilution), DAPI-stained nucleus of MCF-7 and MDA-MB-231 cells. Scale bars, 20 μm.