The effect of HMGB1 and HMGB1∆C on RAGE mRNA and protein expression in breast cancer cell lines: Panel A, MCF-10A; Panel B, MCF-7 and Panel C, MDA-MB-231. The expression of RAGE was examined by quantitative RT-PCR (gray) and by western blot analysis (white) 4 hours after incubation with 10nM final concentration of HMGB1 and HMGB1∆C. The data are normalized to beta actin. The value of 1 corresponds to the levels of RAGE mRNA or protein in the respective cell line stimulated with glycated bovine serum albumin (10 nM G-BSA) as a model protein of AGEs. The data represent the mean SD (n = 5). The representative western blots are shown under the corresponding column charts. The statistical significance of the results was estimated by Student’s t test - * 0.05 > P >0.01 and ** p value is 0.01 > P > 0.005 the stimulatory effect of HMGB1∆C on MCF-10A cells did not differ statistically from the untreated control.