BioDiscovery : Conference Abstract
Conference Abstract
Basal and PGF-stimulated secretion of pro-inflammatory cytokines from 3T3-L1 adipocyte-like cells
expand article infoBilyana Ilieva, Elena Marinova§, Hristo Gagov|, Rossitza Konakchieva
‡ 1 Sofia University St. Kliment Ohridski, Faculty of Biology, Sofia, Bulgaria
§ MC ReproBioMed Ltd, Sofia, Bulgaria
| Sofia University St. Kliment Ohridski, Faculty of Biology, Sofia, Bulgaria
Open Access


Adipocytes were recently identified as an important source of endocrine and paracrine mediators, regulating the metabolism and activity of various cell types and body functions. 3T3-L1 preadipocytes are useful model for physiological, pharmacological and cell signaling studies. Differentiation of 3T3-L1 murine fibroblasts into adipocyte-like cells was conducted in presence of IBMX, dexamethasone and insulin and demonstrated by Oil Red O staining of accumulated lipid droplets. Using Inflammatory Multi- Analyte Cytokines ELISArray Kit we investigated the release of cytokines under basal conditions, after PGF treatment for 24 hours to induce pro-inflammatory phenotype, and after PGF treatment and incubation in the presence of L-C-Propargylglycine (PGG, 1 mmol/l), a selective inhibitor of cystathionine-gamma-lyase (CSE). The last combination was used to explore the role of H2S, released from CSE, for cytokine and H2O2 release. We found that PGF strongly increased TNFα secretion from differentiated adipocytes, the latter effect being antagonized by PGG. The CSE inhibitor enhanced IL-6 production and suppressed IL-10 secretion. PGG enhanced H2O2 production of in PGF-treated cells. It is concluded that pro-inflammatory phenotype of differentiated 3T3-L1 adipocyte-like cells, induced by PGF is characterized by enhanced TNFα production which critically depends on the ability of CSE to produce H2S. 


adipocytes, paracrine mediators, inflammation, hydrogen sulfide, cystathionine-gamma-lyase (CSE) 

Presenting author

Bilyana Ilieva

Presented at

World BioDiscoivery Congress 2017


This work was supported by SU Scientific Research Fund, project №: 80-10-17/12.04.2017 

Conflicts of interest


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