BioDiscovery : Conference Abstract
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Corresponding author: Marina Hristova (mhristova_@abv.bg)
Received: 14 Aug 2017 | Published: 21 Aug 2017
© 2017 Marina Hristova, Elena Hristova, Plamen Todorov
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Hristova M, Hristova E, Todorov P (2017) Osteogenic, adipogenic and neurogenic differentiation of human adipose-derived mesenchymal stem cells before and after cryopreservation. BioDiscovery 20: e20272. https://doi.org/10.3897/biodiscovery.20.e20272
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Mesenchymal stem cells (MSCs) represent a promising tool for the regeneration of damaged tissues in cell therapy. They are characterized as undifferentiated progenitors, which have the ability for self-renewal and multilineage differentiation potential. The development of effective protocols for long-term storage, with the aim of subsequent clinical usage, is essential for their application as cellular therapeutics. In our experiments, we aimed to investigate fresh and cryopreserved human adipose tissue-derived MSCs (AT-MSCs) and their osteogenic, adipogenic and neurogenic differentiation potential.
The obtained results pointed out that the cells possess spindle-like shape and form characteristic wave-like layers when reaching confluency. The immunophenotypic analysis shows that they express CD73, CD90 and CD105 and lack the hematopoiеtic lineage markers CD34 and CD45. Both cryopreserved and fresh hAT-MSCs maintain similar ability to specialize towards osteogenic, adipogenic and neural lineage. In conclusion, the present study indicates that the isolated cells are cryotolerant and are able to retain their morphological and immunophenotypical characteristics after freezing, as well as their multilineage differentiation potential.
mesenchtmal stem cells, cryopreservation, differentiation
Marina Hristova
The current research was partially supported by the “Programme for career development of young scientists, BAS”, projects DFNP-173.
Sofia University "St. Kliment Ohridski"